The Utilization of Milk as a Catalyst Material in Enzyme-Mediated Calcite Precipitation (EMCP) for Crack-Healing in Concrete
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https://doi.org/10.9744/ced.23.1.54-61
Keywords:
Cracking, compressive strength, EMCP, microbes, permeabilityAbstract
This study discussed the applicability of the calcite precipitation method to repair the concrete's crack. The grouting solution of Enzyme-Mediated Calcite Precipitation (EMCP) was modified by adding milk as a catalyst in calcite formation. Cracks in concrete samples were made when the concrete was 28 days with a width of 0.1-0.3 mm. The EMCP solution composed of urease, urea, CaCl2, and milk was injected into the cracked concrete sample, and its effect on permeability and compressive strength tests were evaluated. The result shows that the optimum composition of milk used in the formation of calcite had a concentration of 5 g/L with an initial preparation temperature of 70oC, which produced 26% higher than the initial EMCP solution. The mechanical test results show that the reduction of coefficient of permeability of 92.23% compared to the cracked sample and the improve strength up to 98.75% of the non-cracked sample were obtained by three circles injection. This study elucidated that milk utilization as a catalyst material in repairing cracks with the EMCP method is a potential method for crack-healing concrete.References
W. Khaliq and M. B. Ehsan, “Crack healing in concrete using various bio influenced self-healing techniques,” Constr. Build. Mater., vol. 102, pp. 349–357, 2016.
H. Hartono, “Analisis Kerusakan Struktur Bangunan Gedung Bappeda Wonogiri,” Din. Tek. SIPIL, vol. 7, no. 1, pp. 63–71, 2007.
F. F. Alrizal, J. J. Ekaputri, and Triwulan, “Pemanfaatan pozolan alam sebagai bahan komposit semen dan penggunaan kapur sebagai mineral untuk self-healing concrete,” vol. 1, no. 1, pp. 1–5, 2013.
H. Mihashi and T. Nishiwaki, “Development of engineered self-healing and self-repairing concrete-state-of-the-art report,” J. Adv. Concr. Technol., vol. 10, no. 5, pp. 170–184, 2012.
W. Herlambang and A. Saraswati, “Bio Concrete : Self-Healing Concrete, Aplikasi Mikroorganisme Sebagai Solusi Pemeliharaan Infrastruktur Rendah Biaya,” Simp. II – UNIID 2017, no. March, pp. 520–524, 2018.
J. Bashir, I. Kathwari, A. Tiwary, and K. Singh, “Bio Concrete- The Self-Healing Concrete,” Indian J. Sci. Technol., vol. 9, no. 47, 2016.
V. Achal, A. Mukherjee, and M. S. Reddy, “Microbial concrete: Way to enhance the durability of building structures,” J. Mater. Civ. Eng., vol. 23, no. 6, pp. 730–734, 2010.
T. H. Nguyen and E. Ghorbel, “Effects of Bacillus Subtilis on the compressive strength, porosity and rapid chloride permeability of concrete,” Rilem, vol. 37, no. c, pp. 223–228, 2019.
and M. O. H. Yasuhara, K. Hayashi, “Geo-Frontiers 2011 © ASCE 2011 4762,” Public Work., no. Ara 2004, pp. 4762–4772, 2011.
W. Suwito, “Bakteri yang sering mencemari susu: deteksi, patogenesis, epidemiologi, dan cara pengendaliannya,” J. Litbang Pertan., vol. 29, no. 3, pp. 96–100, 2010.
H. K. Silver, “Sterilization and preservation of formulas for infants,” vol. 20, no. 6, pp. 993–999, 1957.
[BSN], SNI 03-1970-1990, Metode Pengujian Berat Jenis dan penyerapan air agregat halus. 1990.
[BSN], SNI 03 – 1971 – 1990, Metode pengujian kadar air agregat. 1990.
[BSN], SNI 03-6820-2002, Spesifikasi Agregat Halus Untuk Pekerjaan Adukan dan Plesteran Dengan Bahan Dasar Semen, 2002.
[BSN], SNI 2417:2008, Standar Cara uji keausan agregat dengan mesin abrasi Los Angeles. 2008.
[BSN], SNI 03-2834-2000, Tata Cara Pembuatan Rencana Campuran Beton Normal. 2000.
H. Putra, H. Yasuhara, N. Kinoshita, D. Neupane, and C. W. Lu, “Effect of magnesium as substitute material in enzyme-mediated calcite precipitation for soil-improvement technique,” Front. Bioeng. Biotechnol., vol. 4, no. 37, pp. 1–8, 2016.
D. Neupane, H. Yasuhara, N. Kinoshita, and T. Unno, “Applicability of enzymatic calcium carbonate precipitation as a soil-strengthening technique,” J. Geotech. Geoenvironmental Eng., vol. 139, no. 12, pp. 2201–2211, 2013.
D. Neupane, H. Yasuhara, H. Putra, and N. Kinoshita, “Inorganically Precipitated Phosphates and Carbonates to Improve Porous Material Properties,” EPI Int. J. Eng., vol. 1, no. 1, pp. 1–6, 2018.
SNI 1974, “Cara Uji Kuat Tekan Beton dengan Benda Uji Silinder,” Badan Stand. Nas. Indones., p. 20, 2011.
H. L. T. Mobley and R. P. Hausinger, “Microbial ureases: Significance, regulation, and molecular characterization,” Microbiol. Rev., vol. 53, no. 1, pp. 85–108, 1989.
H. T. Amallia, “Pengaruh Cara Penyajian Dan Lamanya Waktu Pajanan Terhadap Kualitas Susu Formula Anak-Anak,” Biota, vol. 3, no. 1, p. 43, 2017.
M. Purwanti, M. Sudarwanto, W. P. Rahayu, and S. A. Winny, “The growth of Bacillus cereus and Clostridium perfringens spores under a variety of preparation and storage condition,” Teknol. dan Ind. Pangan, vol. XX, no. 1, pp. 1–8, 2009.
N. J. Rowan, J. G. Anderson, and A. Anderton, “Bacteriological quality of infant milk formulae examined under a variety of preparation and storage conditions,” J. Food Prot., vol. 60, no. 9, pp. 1089–1094, 1997.
N. Gopal, C. Hill, P. R. Ross, T. P. Beresford, M. A. Fenelon, and P. D. Cotter, “The prevalence and control of Bacillus and related spore-forming bacteria in the dairy industry,” Front. Microbiol., vol. 6, no. DEC, pp. 1–18, 2015.
A. Hatmanti, “SPP. oleh Ariani Hatmanti *),” vol. XXV, no. 1, pp. 31–41, 2000.
H. Prabowo, “Persyaratan Durabilitas Beton Struktural Sebagai Langkah Awal Menuju Desain Umur Layan Bangunan Secara Eksplisit,” no. May, pp. 1–7, 2017.
N. Suhana and N. Kania, “Studi eksperimental kuat tekan dan permeabilitas beton normal berpori dengan fatigue,” vol. 1, no. 4, pp. 176–189, 2016.
J. Y. Wang, D. Snoeck, S. Van Vlierberghe, W. Verstraete, and N. De Belie, “Application of hydrogel encapsulated carbonate precipitating bacteria for approaching a realistic self-healing in concrete,” Constr. Build. Mater., vol. 68, pp. 110–119, 2014.
M. Nemati and G. Voordouw, “Modification of porous media permeability, using calcium carbonate produced enzymatically in situ,” Enzyme Microb. Technol., vol. 33, no. 5, pp. 635–642, 2003.
H. M. Farrell, T. F. Kumosinski, E. L. Malin, and E. M. Brown, “The caseins of milk as calcium-binding proteins.,” Methods Mol. Biol., vol. 172, pp. 97–140, 2002.
H. Nakamura, T. Nanri, T. Miura, and S. Roy, “Experimental investigation of compressive strength and compressive fracture energy of longitudinally cracked concrete,” Cem. Concr. Compos., vol. 93, no. June, pp. 1–18, 2018.
J. O. Simajuntak and T. E. Saragi, “Jurnal Poliprofesi-Hubungan Perawatan Beton dengan Kuat Tekan.pdf.” pp. 1–6, 2015.
S. S. Bang, J. K. Galinat, and V. Ramakrishnan, “Calcite precipitation induced by polyurethane-immobilized Bacillus pasteuri,” Enzyme Microb. Technol., vol. 28, no. 4–5, pp. 404–409, 2001.
H. J. Chen, M. C. Chen, and C. W. Tang, “Research on improving concrete durability by biomineralization technology,” Sustain., vol. 12, no. 3, 2020.
H. J. Chen, C. F. Peng, C. W. Tang, and Y. T. Chen, “Self-healing concrete by biological substrate,” Materials (Basel)., vol. 12, no. 24, pp. 1–16, 2019.
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